Nucleic Acid Modifications

Bio-Synthesis Inc ( Texas )

 

Custom Nucleic Acid Synthesis--"FASTEST services in the industry"

Nucleic Acid Modifications

 

 

オリゴ合成中、または合成後の広範な修飾をおこなうことができます。 Digoxigenin といくつかの蛍光色素については合成中の修飾ができませんが、この場合、合成後にNHSエステル法を使いオリゴに付加させます。

NHSエステルは1級アミンと反応し安定な共有結合のアタッチメントとなります。1級アミンはそれゆえに、希望するNHSエステルとの反応のため、オリゴ合成中にオリゴに加えられます。

オリゴ合成中の直接修飾に比べ、オリゴ合成後の修飾はその効率が低くなります

全てのNHSエステルの修飾ではHPLC精製が必要となります。PAGEによる精製は収率が悪く、ときに修飾自体を壊してしまうこともあります。

価格については以下のリストをクリックください

Terminus and Internal Modifications

Acridine

Aldehyde

Amino modifiers

Branching

Carboxy modifiers

Chain terminator

Cholesteryl

Dendrimer

Glyceryl

Phosphorylation

Psoralen

Spacer

Thiol

 

Non-radioactive Labelings

Biotin

Digoxigenin

2,4-dinitrophenyl (DNP)

 

Bases with Altered Paring

DNA damage/repair studies

DeoxyUridine (dUracil)

Halogenated Bases

 isoCytosine (iso-dC) and isoGuanosine (iso-dG)

Spiked Oligos

Universal Bases

Degenerate Base – Wobble

 

PCR/Sequencing Utilities

Duplex Effects

Chain Terminators

Bases Increasing Duplex Stability

2'-5' Linked Oligonucleotides

Constrained Nucleotide

2'-O-methyl Modified Oligonucleotides

Methylphosphonate

C-Propyne Analogues

Morpholino Oligonucleotides

C5 - Methyl Analog

Peptide Nucleic Acid (PNA)

Chimeric Oligos

Phosphorothioate

Photo Cleavable

PC-Amino

PC-Spacer

PC-Biotin

PC-Linker

Bioconjugations

We offer a personalized solutions to assist client cross link various molecules and compound and solid support attachment. More...

·         Biomolecule-small molecule labelings

·         Biomolecule-biomolecule conjugations

·         Immobilization on solid supports

Antisense Oligonucleotides

Use antisense oligonucleotides for your gene silencing experiments. We hybrid designs using various types of nucleotide analogs to increase high affinity for a successful knockdown experiments. More....

·         Phosphorothioate oligos

·         Constrained ribose (ENA, ZNA)

·         2' OMethyl Base

·         2' Fluoro RNA

·         Peptide Nucleic Acids (PNA)

·  Peptide Nucleic Acids (PNA)

·  Morpholino

·  5-Me-dC

·  Propyne dC or dU

Quencher

Black Hole QuencherTM

Eclipse® Dark Quencher

DABCYL

TAMRA

Fluorophore Labelings

Alexa Fluor® Dyes

Epoch Dye and Quencher

ATTO-TEC Dyes

Fluorescein Dye

Bodipy® Fluorophores

Oregon Green

Cyanine Dye

Other Molecular Probe Dyes

Dyomic Dyes

Oyster Dye

Rhodamine Dyes

 

Redox Labels

Bio-Synthesis offers labeling of oligonucleotide or peptide/protein with redox active compounds for electrochemical studies.

·         Dicarboxymethylene Blue

·         Monocarboxymethylene Blue

·         Ferrocene, Ferrocene-C6

·         Anthraquinone, Anthraquinone-C6

Fluorogenic Probes and Primers for Real-time qPCR

Labeled Probes

Amplifluor Direct® Primers

Molecular Beacons for Gene Detection and Analysis

Plexor™ Primers

Black Hole Scorpion™ Primers

 

Probes for Multiplexing qPCR

Labeled probes

Amplifluor® Direct Primer

Molecular Beacon

Black Hole Scorpions™ Probe, Uni-molecular

Disclaimer: FAMTM, HEXTM, ROXTM, TAMRATM and TETTM are trademarks of Applied Biosystems, Inc. CyTM is a trademark of GE Healthcare; Texas RedTM is a trademark of Molecular Probes Eclipse® and Yakima Yellow® are registered trademarks of Epoch Biosiences, Inc. BHQ 1TM and BHQ 2TM are trademarks of Biosearch Technologies, Inc. BlackBerry (BBQ)TM is a trademark of Berry & Associates, Inc. LightCycler® probes are sold under the license from Roche Diagnostics Gmbh

                      

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