siRNA合成


siRNA合成サービス
Biosynthesis – DNA, RNA, ペプチド合成のパイオニアとして30年余、そしてBiologicaとも25年以上の実績。

Custom siRNA Synthesis – Road to gene silencing

Bio-Synthesis offers reliable custom siRNA synthesis in ready-to-use duplex multi-scale quantities for your silencing applications. We offer a wide range of modifications and purification methods. Large-scale sterile, endotoxin-free preparations are available specifically for therapeutic applications. You may submit your siRNA sequence of choice, or use our design tool to configure a siRNA sequence. All custom siRNA oligonucleotides are manufactured under strict ISO standards and undergo QC by analytical HPLC, mass spectrometry, annealed in RNase-free water and duplex analyzed by analytical gel. Contact us for custom siRNA synthesis.

Synthetic siRNA and shRNA constructs are just two of the numerous options available. Bio-Synthesis provides synthesis of sequence-specific custom siRNA for direct transfection, as well as synthesis of shRNA oligos, ~60 bases long purified DNA oligos to be cloned to form shRNA constructs. Our siRNAs (small interfering RNAs) are double-stranded RNA molecules, made of up to 30 RNA nucleotides with two nucleotides 3′ overhangs. They are used to knock-down a specific gene of interest for identifying gene function, elucidating pathways and screening for potential new drug targets.

Custom siRNA Services

  • Large variety of modifications
  • Deprotected, desalted, annealed, In-vivo quality prep
  • µg to multi-gram capacity for ASR or cGMP grade
  • Free online design Sci-Apps tools (メーカーサイト)
  • Available in duplex or single-stranded format
  • Standard and advanced QC packages are available

Custom Duplex siRNA Synthesis

Our siRNA oligos are available at a fixed price per duplex oligonucleotide strands of up to 30 nt target sequence from which both the sense and antisense strand will be synthesized, including 3′ overhang bases. Contact us for longer oligos or spiked oligos which are available upon requests, for volume discounts.

Scale Desalted in vivo HPLC In vivo HPLC
量 (n mol) 参考
価格
量 (n mol) 参考
価格
量 (n mol) 参考
価格
量 (n mol) 参考
価格
0.015 µmol 10 25,800円
0.025 µmol 20 32,000円
0.05 µmol 50 40,000円 25 113,000円
0.25 µmol 150 87,100円 100 124,000円 65 117,000円 50 161,000円
0.5 µmol 350 125,000円 225 168,000円 180 159,000円 125 205,000円
1.0 µmol 750 171,000円 500 223,000円 375 212,000円 250 271,000円
2.0 µmol 1500 282,000円 1000 335,000円 750 340,000円 500 413,000円
5.0 µmol 3750 482,000円 2500 535,000円 1875 549,000円 1250 662,000円
10.0 µmol 7500 765,000円 5000 833,000円 3750 884,000円 2500 950,000円
>10 umole Inquire

より大きなスケールの供給も致します。お問い合わせください。
直接納入の場合、10%引きとなります。10万円以上の場合、15%引き、送料別途。
*Guaranteed yields (ODs) and approximate yields (nmols) are for oligos > 20 bases and < 40 bases *

Sample Processing Options

Processing Types Desalted HPLC In vivo In vivo HPLC
Desalted X X X X
Deprotected X X X X
Duplexed X X X X
Purified X X
Endotoxin tested X X
Sodium counter-ion X X
Recommended for modified RNA X X
Recommended for in vivo use X X
  • Desalted Single-Stranded:
  1. Oligos are shipped deprotected, desalted single strand in a separate tube.
  2. Dried duplexes are provided in the absence of universal buffer salts.
  3. Oligos are ready for use immediately upon resuspension.
  4. Estimated shipping time is 4 business days for orders received before 3:00pm CST. Ship date will be confirmed upon order placement.
  • Desalted Duplex strands:
  1. Duplex oligos are shipped deprotected and desalted.
  2. Dried duplexes are provided in the absence of universal buffer salts.
  3. The oligos are ready for use immediately upon resuspension.
  4. Estimated shipping time is 4 business days for orders received before 3:00pm CST. Ship date will be confirmed upon order placement.
  • HPLC Purified:
    Please Note: The siRNA duplex is now dried down in the absence of buffer and the amount packaged per tube has changed, however, the total amount of material supplied remains the same.
  1. Oligos are shipped deprotected, duplexed, purified, and desalted.
  2. Dried duplexes are provided in the absence of universal buffer salts, unless otherwise specified upon ordering.
  3. The oligos are ready for use immediately upon resuspension.
  4. Typical shipping time is 6 business days for orders received before 3:00pm CST. Ship date will be confirmed upon order placement.
  • siRNA In-Vivo-Ready: A minimum of 90% purity. These duplexes are shipped deprotected with extra processing which also includes counter ion (Na+) exchange, dialysis, sterile filtration and endotoxin testing for the introduction of siRNAs into animals.Estimated shipping time is 10 business days for orders received before 3:00pm CST. Ship date will be confirmed upon order placement.
  • HPLC purified: In-Vivo-Ready custom synthesized siRNAs provide the highest quality siRNAs available anywhere with guaranteed purity of 97%. Pprocessing includes an extra level of HPLC purification, counter ion (Na+) exchange, dialysis to remove salt (to <1 microsiemens conductance at 50 µM siRNA), sterile filtration and endotoxin tested.Estimated shipping time is 20 business days for orders received before 3:00pm CST. Ship date will be confirmed upon order placement.

Modifications and Labelings

Custom siRNA is available with a variety of fluorescent labels and other modifications. Alexa Fluor dyes provide superior performance and can be directly substituted for other common dyes. Other fluorescent dyes available include fluorescein, rhodamine, Cy3, and Cy5. Custom siRNA may be labeled at either the 3′ or the 5′ end of the sense strand, and labels do not affect the biological activity of the siRNA. As well as fluorescent labels, many other backbone and base modifications are available including amino linkers, thio linkers, biotin, dabcyl and phosphate modifications. See the full range of our modification options. Special modifications are also available upon request. You may contact us for additional information.

Note: all siRNA oligos are provided as lyophilized pellets to ensure stability during delivery.

Synthesis Platform

Bio-Synthesis provides siRNA by through proprietary synthesis and purification processes with final products that are ready-to-use. There is no need for further time-consuming and expensive HPLC or PAGE purification. The high-throughput synthesis and purification processes enable fast turnaround and high yields. Each duplex siRNA synthesis undergoes stringent quality control including MALDI-TOF mass spectrometry analysis that verifies identity of the component strands and analytical HPLC QC to verify purity of the duplex itself, a level of QC unparalleled in the industry. Our high performance grade siRNA is economically priced allowing the use of highly pure siRNA in all routine RNAi experiments. For very sensitive applications, custom synthesis of HPLC-purified siRNA is also available upon request.

Quality Control Options

Standard QC package

Analytical anion-exchange HPLC of oligonucleotide; purity guaranteed above 85% in case purification is required. TOF/MS for oligonucleotide identification.

Advanced QC package

Standard QC package and additionally:

Ion-pair reverse phase HPLC of the oligonucleotide at 60oC, purity guaranteed above 85% analytical reverse phase HPLC Endotoxin analysis of the oligonucleotide (*phosphorothioates may inhibit in the mechanism for endotoxin determinations Bio- Synthesis is using and therefore reading might be less accurate for these molecules) pH determination of a 1% oligonucleotide solution.

Custom QC Package:

Determination of the sodium content of the oligonucleotide (minimum 20 mg of purified oligonucleotide is required). LC-MS of the oligonucleotides. Any custom quality control methods could be developed.