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Globin mRNA is known to be over 70% of the total mRNA in blood cells that lead to low level of sensitivity for detection of non-globin mRNA. PNA oligomers can be effectively used as a clamp by specifically blocking globin mRNA during the process of reverse transcription and resulting in specific PCR amplification of the target non-globin mRNA for your analysis.
PNA oligomers for PCR clamping of Globin Reduction