InstantAgarose™

DNA電気泳動用インスタントアガロース

InstantAgarose™ Tablet Gel Preparation Protocol & 仕様

  1. Use a flask that is 2 to 4 times the volume of the solution being prepared. IMPORTANT
  2. Add agarose tablet to electrophoresis buffer based on below table to prepare your desired gel %, soak tablet in buffer for 2 minutes before heating.
  3. If you use boiling water bath:
    • To melt agarose, simply heat the slurry in a boiling water bath, bring the solution to a boil and allow it to boil for 5-10 minutes stirring continuously, until agarose dissolves completely.
  4. If use microwave oven:
    • Heat the slurry in microwave on high power setting until it starts to boil, allow boiling for 30 seconds.
    • Remove the flask from microwave, swirl gently to re-suspend any remaining agarose particles.
    • Reheat on high power for 1-2 minutes or until the solution is clear and all particles are dissolved.
    • Remove the flask from the microwave oven, and gently swirl to it. Use caution when handling as solution may be extremely heated.
  5. Cool the solution to approx. 60°C before pouring. Separation of DNA in agarose.
product Instant Agarose
CAS
appearance White to off-white
EEO ≤ 0.13
Gelling point 36°C±1.5°C (1.5% gel)
Melting point 88°C±1.5°C (1.5% gel)
solubility 1g in 100ml water
moisture ≤ 10%
Gel strength ≥ 1200 g/cm2 (1% Gel) 
Sulfate ≤ 0.15%
Ash ≤ 0.5%
Impurities None Detected