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目次

ナノボアツール FSチューブ 各種フィッティング 前処理用
Trap’n TipTM		 参考価格

NonoHPLC関連の必需品

Nanobore Tools and Hardware

Close-up photo of diamond scribe

Diamond Scribe

This precision, 90° wedge-style (1 mm-wide blade) diamond scribe is different from most diamond point scribes available elsewhere. It’s the tool of choice by our own production techs. With proper technique, this scribe can be used to cleave even thin-wall tubing without damage and with minimum particulate generation.

Nano-LC/MS用溶融石英管をうまく切る方法

Part Number: SCRIBE
Photo of micro tool kit

Micro Tool Kit

Tool assortment for low-volume preparative work. These high-quality tools are the same as those used in the New Objective lab. Includes Dumont #7 fine forceps, Dumont #578A forceps, 90° precision diamond scribe, needle probe for swaging sleeves, and a 6″ metal ruler (inch/mm).

Part Number: TIP-KIT
Photo of micro tool kit

PicoFrit Packing Mount

Acrylic base for the inspection of fused-silica tips by light microscopy; especially useful for holding PicoFrit tips during packing. The 1″ x 3″ baseplate is designed to fit any conventional mechanical stage. Includes MicroTight union and ferrules for 360 µm OD fused-silica tubing. Ideal for use with the PIP-500 Injection Platform.

Part Number: MOUNT-FS1

Fused-Silica Tubing

Pre-Cut Tubing

Simplify and improve your analysis with pre-cut fused-silica tubing. No more cleaving and hoping for a clean connection. Pre-cut tubing is professionally cut and polished to guarantee the best possible connection. 360 µm OD tubing is available in four ID sizes and five lengths.


Machine Cut
Machine cut and polished ensures fast, no-fuss, clean connections everytime
Good Hand Cleave
Good technique with diamond scribe or using a rotary cutter produces relatively good cuts
Poor Cleave
Usually results from ineffective scoring of fused silica and/or bending the tubing to “snap” it
Poor Cleave
Crushed tubing results from too much pressure and poor technique or inappropriate cutting tool

Ordering Pre-Cut Tubing

Substitute your desired tubing ID for the ## in the part number when ordering.

OD/ID µm ID µm Length
cm		 Qty 型番
360 20, 50, 75, 100 6 5 CT360-##-6-5
360 20, 50, 75, 100 10 5 CT360-##-10-5
360 20, 50, 75, 100 15 5 CT360-##-15-5
360 20, 50, 75, 100 25 5 CT360-##-25-5
360 20, 50, 75, 100 50 5 CT360-##-50-5
Pre-Cut Super Pack:
Contains 25 pieces of same size ID; five (5) pcs. each of all five (5) lengths		 CT360-##-SP

Stock Fused-Silica Tubing

Fused-silica tubing is essential for making connections from your HPLC system through your source. This high-grade tubing is the same tubing used to produce our columns and tips. Shipped in 2 m lengths, our fused silica is available with a 360 µm OD in four different ID sizes.

OD/ID µm ID µm Length
m		 Qty 型番
360 20 2 1 RT360-20
360 50 2 1 RT360-50
360 75 2 1 RT360-75
360 100 2 1 RT360-100

各種フィッティング

Nanobore Fittings

Fittings Kit

Everything you need for connecting fused-silica tubing with conventional HPLC hardware, Luer lock, and injection syringes. Kit includes:

Order Number: FSFK-2
  • (1) M-531-1 Microfilter Assembly
  • (1) M-121-1 Filter Cap Assembly, 4 pk
  • (1) P-770 ZDV PEEK Adapter
  • (1) F-172 MicroFerrule, .025″, 4 pk
  • (1) P-720 MicroTight Union
  • (1) F-125 Micro FingerTights, 4 pk
  • (1) P-775 MicroTee Assmebly, .025″
  • (1) F-183X MicroTight Sleeves, 280 µm, 10 pcs
  • (1) F-185X MicroTight Sleeves, 395 µm, 10 pcs
  • (1) F-240X SealTight Sleeves, 280 µm x 1/16, 10 pcs
  • (1) F-242X SealTight Sleeves, 380 µm x 1/16, 10 pcs
  • (1) F-331NX FingerTight w/ferrules, 10 pk
  • (1) P-659 10-32 Female-to-Female Luer Adapter
  • 2m lengths each of 360/20, 360/50, 360/75, and 360/100 µm OD/ID fused-silica tubing

Upchurch® Fittings & Connectors

型番 Description Qty
NOF-125x Std. Head Fittings for MicroTight® Sleeves, Natural PEEK™ 10
NOF-152 MicroFerrule for 360 µm OD tubing, Natural PEEK™ 1
NOF-172x MicroFerrule for .025″ OD Tubing, Black PEEK™ 10
NOF-184x MicroTight®PEEK™ Tubing Sleeves, 330 µm ID, Orange 10
NOF-185x MicroTight®PEEK™ Tubing Sleeves, 395 µm ID, Green 10
NOF-242x SealTight™ FEP Sleeves 1/16″ OD, 395 µm ID, Green 10
NOF-331Nx NanoTight Nuts 10-32 w/F-142N Ferrule, Natural PEEK™ 10
NOF-241x SealTight™ FEP Sleeve 1/16″ OD, 335 µm ID, Orange 10
NOP-720 True ZDV Union w/Fittings & Gauge Plug, Natural PEEK™ 1
NOP-775 MicroTee, 150 µm Thru-holes, PEEK™ 1
NOP-777 MicroCross for MicroTight® Sleeves (150 µm Thru-holes) 1
NOM-120x PEEK™ End-Fittings for Inline MicroFilter with .5 µm Frit 10
NOM-134 1 µm NanoFilter capsule, PEEK™ w/Titanium Frit, 1/32″ 2
NOM-520 Inline Microfilter Assembly w/Fittings, .5 µm 1
NOM-538 Inline NanoFilter Assembly for 360 µm Tubing w/Fittings 1

Valco Fittings & Connectors

型番 Description Qty
NOZU1TTI Zero Dead Volume Titanium Union 1/16″ 1
NOC-NNFLFPK Nanovolume Nut & Ferrule for 10-port valve 1

Sample Prep Essentials

Trap’n Tips™ Sample Prep Tips

蛋白質・ペプチドサンプルの濃縮、精製チップ、MS測定前の簡易調製に最適です。
Trap’nTipTM (トラップンチップ)は内壁に吸着素材をコートしたピペットチップで内壁に目的の溶質を保持する濃縮・精製チップです。
C18、Carbon、C18/Carbon、Titanium、Zirconiumの5種類の吸着素材が用意されています。
Zirconium素材のものは燐酸化ペプチドの洗浄と脱塩にオフラインMS測定前に使用することをお奨めします。

Trap'nTips
Carbon-, C18/Carbon-, and C18-coated Trap’nTips		 Trap’nTip™ ready-made, disposable pipette tips with micro- chromatography columns are perfect for sensitive, fast, and convenient purification of peptide mixtures and proteins. Chromatography material is directly attached to the inner surface of the tip without the use of any polymers or glue.

  • Excellent sensitivity: フェムトモルからアトモルの狭間での利用
  • High reproducibility: チップバラつきがなく 使用が簡単、信頼性の高い使用法です
  • High throughput: 吸着・洗浄・溶出を高速で(>300 µL/min)、バックプレッシャーは低いのです
  • Sample capacity: 最大0.5 µg、最小20 nLのサンプルにも対応できます
  • Applications: 脱塩、予備濃縮、ステップワイズ溶出 (MALDI, LC-MS向けの簡易分離)、ナノエレクトロスプレー (エミッターへの直接注入)
Trap'nTip on pipetter
Trap’nTip filled with sample on a standard pipetter		 型番 Sorbent Qty/Box
NOTNTC18 C18 96
NOTNTCAR Carbon 96
NOTNTMC18 Carbon/C18 96
NOTNTTI Titanium 96
NOTNTZR Zirconium 96

Trap’nTipの参考価格はこちら

その他のサンプル前処理用ツール

Hamilton Syringe
Hamilton syringe
10 µL syringe with a 10 cm long, 170 µm OD flexible fused-silica needle. Ideal for filling offline PicoTips.
Order Number: NO14-824		 Calibrated Micropipettes
Calibrated pipettes
Precision-calibrated 1-5 µL micropipettes are ideal for monitoring offline sample loading of PicoFrit columns. Fire-polished and color-coded with a guaranteed accuracy of ±0.5%, these are ideal for measuring through-column flow rates.
Order Number: NO2-000-001
250 pieces per box		 Eppendorf GELoader tips
Eppendorf GelLoader tips
Flexible 350 µm OD gel-loading pipette tip, makes offline PicoTips® sample loading fast and simple.
Order Number: NO22351656
Box of 192 tips		 Glygen NuTips
NuTips
Ready-made, disposable pipette tips with C18 micro-columns for fast, simple purification of peptide mixtures and proteins. Perfect for MALDI and offline applications.
Order Number: NONT1C18
Box of 192 tips
下記リンク先はメーカーページです。

More Information

Using the right tools for making fused-silica connections can mean the difference between frustration and success.

Cleave fused silica using the proper diamond-bladed tool and with proper technique to ensure particulate-free connections. Or use pre-cut emitters and tubing for trouble-free connections.

New Objective社製品のお問い合わせ・ご注文