ナノHPLCカラム : LC/MS

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目次

New Objective製ナノボアカラムの特長

HALOの fused-core 粒子は固体シリカ粒子の上で多孔質シリカ膜を溶かすことにより作られます。HALO粒子は径分散が少なく、ピークの広がりを最小化します。これは400DA以上の分子のクロマトグラフィーと、より流速の早い高速分離にとって特別に有利なことです。

以下のクロマトグラムはHALOを充填したトラップ、分析カラムのコンビネーションで30fmolと300fmolのBSA消化物をインジェクションし再現性を見るためのものです。2-50%Bのリニア勾配を使用。30fmolと300fmol分析の前に3回、ブランクランを実施。

カラム詳細パンフレット

カラム ラインナップ

  1. NanoLCのカラムはNew Objectiveのまずは、インテグラフリットカラム、ピコフリットカラムの2種類。
    • インテグラフリットカラムは従来仕様のナノカラムですが、漏れ防止のフリットがしっかりしたナノカラムで充填剤の種類の多さ、価格の安さで他社を圧倒しています。
    • ピコフリットカラムはカラムがスプレーチップであるという、直接イオン化タイプのカラムです。分離、即、イオン化ということでデッドボリュームによる分離ピークの拡散を防ぎ、クロマト時間を短縮します。なぜか耐久性に優れ500回分析の実績が紹介されています。カラム中の充填剤が常に圧力勾配のある電場に置かれることによる蛋白質の吸着を防ぐ効果があるのでしょうか。
    • セルフパック用のエンプティーカラムも用意しています。お好きな充填剤を、お好きな径でお好きな長さに充填することができます。
  2. カセット式直接イオン化用スプレーカラム”ピコチップ”も。

詳細は以下のリンク先ページをご覧ください。

PicoChip™

PicoFrit® & SelfPack
カセット式直接イオン化用スプレーカラム
The ultimate performance nanospray column, PicoChip™ columns provide Plug & Spray™ operation with the high-performance and flexibility of traditional nanospray. Single connection, onboard high-voltage connection and a full menu of chemistries, the PicoChip is nanospray at it’s easiest.		 直接イオン化用スプレーカラム
&自分で充填するセルフパック空カラム
PicoFrit® nanospray columns ensure the highest possible performance, combining powerful chemistries with a novel format that eliminates post-column loss. Designed and tested for applications that require optimal peak shape and ultimate sensitivity, these columns provide unsurpassed performance, reliability and ruggedness.

IntegraFrit™ & SelfPack

Sample trap column (pre column)
通常のナノカラム
&自分で充填するセルフパック空カラム
IntegraFrit™ columns are the building blocks of your high performance analytical method. The exceptional flexibility, consistency and stability are the hallmark of these non-metal fritted columns, and allow the full potential of nanospray to be harnessed with the benefits of superior control and performance independent of your analysis platform.		 IntegraFrit™サンプルトラップカラム
IntegraFrit™ sample traps are perfect combination for concentrating and separating samples for mass spectrometry. Integrated with a titanium inlet frit in the cartridge, the IntegraFrit sample trap column features a non-metal outlet frit in 360 µm ID fused-silica tubing. IntegraFrit sample trap columns can be packed with any of our outstanding chemistries.

In-House Chemistries

New Objective社の高品質なProteoPep™ブランドを含む幅広い吸着剤オプションを提供しています。ご希望の品が見当たらない場合は、お問い合わせください。

Order Code Chemistry Name Type, Particle, Pore Size Manufacturer
H001 ProteoPep™ C18, 5 µm, 300 Å New Objective
H002 ProteoPep™ II C18, 5 µm, 300 Å New Objective
H003 ProteoPep™ III C18, 1.8 µm, 80 Å New Objective
H020 Strong Cation Exchange SCX, 5 µm, 85 Å New Objective
H035 HALO® C18, 2.7 µm, 90 Å AMT, Inc.
H036 HALO® C8, 2.7 µm, 90 Å AMT, Inc.
H037 HALO® RP 2.7 µm, 90 Å AMT, Inc.
H038 HALO® HILIC 2.7 µm, 90 Å AMT, Inc.
H039 HALO® Peptide ES C18, 2.7µm, 160 Å AMT, Inc.
H044 HALO® Peptide ES C18, 5µm, 160 Å AMT, Inc.
H040 HALO® Penta HILIC C18, 2.7µm, 90 Å AMT, Inc.
H041 HALO® Protein C4, 3.4µm, 400 Å AMT, Inc.
H042 HALO® Peptide CN CN, 2.7µm, 160 Å AMT, Inc.
H043 HALO® Peptide CN CN, 5µm, 160 Å AMT, Inc.
H050 BioBasic® C4, 5µm, 300Å Thermo Scientific
H051 BioBasic® C8, 5µm, 300Å Thermo Scientific
H052 BioBasic® C18, 5µm, 300Å Thermo Scientific
H053 AQUASIL C18, 5µm, 100Å Thermo Scientific
H054 Betasil® C18, 5µm, 100Å Thermo Scientific
H056 BioBasic SCX SCX, 5µm, 300Å Thermo Scientific
H060 BetaBasic® C8, 5µm, 150Å Thermo Scientific
H061 BetaBasic® C18, 5µm, 150Å Thermo Scientific
H062 Hypercarb C100, 5µm, 250Å Thermo Scientific
H063 Hypercarb C100, 7µm, 250Å Thermo Scientific
H064 Accucore C18, 2.6µm, 150Å Thermo Scientific
H070 Hypersil GOLD® C18, 5µm, 175Å Thermo Scientific
H071 Hypersil GOLD® C18, 1.9µm, 175Å Thermo Scientific
H072 Hypersil GOLD PFP™ PFP, 5µm,175Å Thermo Scientific
H076 Hypersil GOLD PFP™ PFP, 3µm,175Å Thermo Scientific
H075 Hypersil GOLD PFP™ PFP, 1.9µm,175Å Thermo Scientific
H073 Hypersil GOLD aQ™ C18, 1.9µm, 175Å Thermo Scientific
H074 Hypersil GOLD aQ™ C18, 5µm, 175Å Thermo Scientific
H080 ProntoSIL C18 AQ, 3µm, 200 Å
Comparable to Magic AQ		 Bischoff
H100 XBridge™ C8, 3.5µm, 135Å Waters
H101 XBridge™ C8, 5 µm, 135Å Waters
H102 XBridge™ C18, 3.5µm 135Å Waters
H103 XBridge™ C18, 5 µm, 135Å Waters
H110 XTerra® C18, 5 µm, 125Å Waters
H111 Acquity® BEH C18, 1.7 µm, 130Å Waters
H200 Inertsil® ODS-3™ C18, 3µm, 100Å GL Sciences
H201 Inertsil® ODS-3™ C18, 5µm, 100Å GL Sciences
H202 Inertsil® ODS-4™ C18, 2µm, 100Å GL Sciences
H203 Inertsil® ODS-4™ C18, 3µm, 100Å GL Sciences
H204 Inertsil® ODS-4™ C18, 5 µm, 100 Å GL Sciences
H252 MAGIC AQ® C18, 5µm, 200 Å Michrom
H254 MAGIC AQ® C18, 3µm, 100 Å Michrom
H255 MAGIC AQ® C18, 5µm, 100 Å Michrom
H301 POROS-R2 20 µm, 2000 Å
This material is suitable only for column IDs ≥100µm		 Life Technologies/ABI
H302 POROS-R2 10 µm, 2000 Å Life Technologies/ABI
H340 PepMap C18 3 µm, 100 Å Dionex
H354 Reprosil-PUR C18-AQ, 3 µm, 120 Å Dr. Maisch
H355 Reprosil-PUR C18-AQ, 3 µm, 200 Å Dr. Maisch
H356 Reprosil-PUR ODS-3 ODS-3, 3 µm, 300 Å Dr. Maisch
H357 Reprosil-PUR C18-AQ, 5 µm, 120 Å Dr. Maisch
H358 Reprosil-PUR C18-AQ, 5 µm, 200 Å Dr. Maisch
H359 Reprosil-PUR ODS-3 ODS-3, 3 µm, 200 Å Dr. Maisch
H363 Reprosil-PUR C18-AQ, 1.9 µm, 120 Å Dr. Maisch
H401 Monitor® C18, 3 µm, 100 Å Orochem Technologies
H402 Monitor® C18, 5 µm, 100 Å Orochem Technologies
H403 Gazelle™ C18, 1.7 µm, 100 Å Orochem Technologies
H501 PLRP-S 5µm, 4000Å Varian
H502 PLRP-S 5µm, 1000Å Varian
H601 Chromegabond C18, 1.8 µm, 120 Å ES Industries
H602 Chromegabond C18, 3 µm, 120 Å ES Industries
H603 Chromegabond C18, 5 µm, 120 Å ES Industries
H604 Epic C18-MS C18, 1.8 µm, 120 Å ES Industries
H605 Epic C18-MS C18, 3 µm, 120 Å ES Industries
H606 Epic C18-MS C18, 5 µm, 120 Å ES Industries
H607 Epic Polar C18 C18, 1.8 µm, 120 Å ES Industries
H608 Epic Polar C18 C18, 3 µm, 120 Å ES Industries
H609 Epic Polar C18 C18, 5 µm, 120 Å ES Industries
H610 Epic PFP-LB C18, 1.8 µm, 120 Å ES Industries
H611 Epic PFP-LB C18, 3 µm, 120 Å ES Industries
H612 Epic PFP-LB C18, 5 µm, 120 Å ES Industries
H613 ProlyxPore Phenyl SD Phenyl, 5 µm, 220 Å ES Industries
H614 AquaSep WP, 3 µm, 100 Å ES Industries
H615 MacroSep Shell C18, 5 µm, 300 Å ES Industries

お問い合わせください

  • Don’t see the particular chemistry you need?
  • Custom packing is available with just about any chemistry, bed length, or column/tip dimensions you may need.

BULK MATERIAL

New Objective does not provide bulk material. The media listed here is for ordering pre-packed columns only. To purchase bulk media, please contact the media manufacturer directly.

参考価格

New Objective社製品のお問い合わせ・ご注文