ESIスプレーチップ (ナノスプレー) : LC/MS

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目次

• Fossil Ion Technology社エミッタ
• ヒューマニクス社エミッタ

フォッシルイオンテクノロジー社のSHARP SINGULARITY Emitters (LOTUS and Classic)

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新しいLOTUSエミッター!! Hydrophobic coating locks the meniscus to the inner diameter of the emitter Better protein ID and reproducibility The LOTUS emitters are built upon the optimized Sharp Singularity geometry Sharp Singularity – 唯一無二のエミッタ Stable and repeatable signals require extremely tight tolerances at the microscopic scale. Our unique micro-machining process produces: extremely sharp emitters constant inner diameter (from 10 to 50 μm ID) very tight tolerances. Know everything about each emitter. Each and every emitter is inspected and photographed. You will get the a quality control and traceability report for every emitter. Pricing | LOTUS brochure | Sharp Singularity Brochure

データ品質を向上させます。
3つのステップで適切なエミッターを選択

1- エミッターの表面化学を選択 (LOTUS または Classic)

LOTUS The LOTUS emitters have a surface treatment that makes them hydrophobic. The liquid does not wet it, and the meniscus is anchored at the inner edge of the tip. For the same geometry, the lotus emitters produce smaller meniscus. This improves ionization efficiency and repeatability. As a weakness, the LOTUS surface treatment can be damaged over time due to the exposition to fluing ions (the tip of the electrospray is a very harsh environment!!). When testing the LOTUS, we recommend rising the voltage slowly to prevent the formation of a corona, that would rapidly erode the LOTUS treatment. The lotus is for you if you are interested in pushing the performance of your set-up to get the best data quality.

Classic: All Sharp Singularity emitters are made of quartz silica. For optimum performance, the inner surface is rinsed with acidified water, but you will need to condition it for your set-up. This will happen automatically in the first two runs of your nLC workflow. When you install a new emitter, we recommend you to pas two standard runs before analyzing your samples. The Classic surface finish is polished and rinsed with acidified water (HPLC grade). Under the influence of the electric field, the liquid wets the glass and the meniscus is anchored at the outer edge of the tip. The tipical voltages required to stabilize the classic emitters is 2.0KV to 2.5kV. The Classic is for you the conservatives.

Download brochure to learn more about the LOTUS effect:

2- エミッターの内径を選択

Selecting the right ID of the emitter is a trade-off decision between spray stability and robustness (smaller IDs provide better performance but are more susceptible to clogging)
For proteomics applications, the most commonly used emitters are 20μm and 10μm. (we can provide emitters from 10μm ID to 50μm ID).
The ideal emitter ID depends on the application and the flow rate:

• for flow raes above 300nlpm, we recommend the 20μm ID;
• for flow rates below 200nlpm, we recommend the 10μm ID;
• for flows in between, we recommend testing both diameters and choosing whichever works best for your set-up and your application.

You can order a pack of of emitters with a mix of IDs to test.

20 um ID

10 um ID

3- エミッターの長さを選択

The Sharp Singularity emitters are straight cut and polished at the back to ensure they sit properly in their fitting. This eliminates imperfection at the back end of the emitter that can induce bubble nucleation, which is a main cause of spray instability. For this reason, we recommend not to cleave the emitters. If you ned a special length, please contact us and we will be happy to assist you to.

Improve the quality of your data, continue learning!: 

The ionization efficiency depends on the size of the 1st generation nano-electrospray droplets, which is defined by the nano jet, which emerges from the electrospray meniscus, whose shape is determined by the emitter. To produce a stable signal, you need to produce a microscopic and stable meniscus. Having the right emitter is a good start. Understanding the physics of electrospray will help you get the best results. Here you can find some material to learn more:
go to Electrospray for proteomics go to the Electrospray academy

Some reasons why reducing the size of the meniscus helps:

• Solvent evaporation is desirable at the droplets but too much evaporation at the meniscus increases the concentration of contaminants reaching the jet. This enhances ion suppression effects and changes the properties of the liquid, affecting the droplet size, and the optimum the flow and voltage conditions. Smaller meniscus means less solvent evaporation.
• Ion evaporation is desirable at the droplets but, at the vicinity of the nano-jet, this further ionize the gas because of high energy collisions induced by the strong electric fields. These gas ions reduce the droplets charge and hence their ionization efficiency. Ion evaporation is enhanced by solvent evaporation. Smaller meniscus means less ion evaporation.
• Corona discharges form in the gas surrounding the meniscus when the voltage is too high. Ions formed at the discharge are attracted to the droplets and reduce their net charge. The voltage required to form an electrospray depends on the meniscus size. Small meniscus means low voltages an no corona discharges.
• Ion transport: Smaller meniscus produce weaker electric fields pushing the ions forward. For this reason, nano-electrospray must be located very close to the MS inlet. The smaller the meniscus, the smaller the emitter to MS inlet distance.

Offline Emitters

• Cellomics Tip
  Cellomics Tipはヒューマニクス社の製品です。

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