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二重特異性抗体作製
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大変お手数ですが以下の質問にお答えください。お答えできる範囲で構いません。

I. Antigen/Parental Antibody Information

A. Target antigen #1

NCBI/UniProt number of target antigen #1

Target antigen #1 full length sequence

Target antigen #1 description (e.g. whether it is a trans-membrane or soluble protein, whether you can provide the recombinant form, etc.)

Do you already have an antibody against target antigen #1?
YesNo
Yesの場合、以下1から8のご質問にお答えください。
1. Specify the method (e.g. hybridoma, phage display, etc.) + exact antigen used for antibody development

2. Specify the species (e.g. human, mouse, etc.) + backbone/format (e.g. IgG1, Fab, scFv, etc.) of the parental antibody

3. Do you already have its sequence?
YesNo
4-1. Did you already express it as recombinant antibody?
NoYes
4-2. If yes, please specify the format(s) used + backbone species + yield obtained for each format?

5-1. Did you already express it as bispecific antibody?
NoYes
5-2. If yes, please specify the format(s) used + yield obtained for each format?

6. Do you know its epitope?

7. Are you happy with the antibody affinity?

8. Do you have only one antibody against target antigen #1 or several antibodies?

B. Target antigen #2 name (if 2 different targets)

NCBI/UniProt number of target antigen #2

Target antigen #2 full length sequence

Target antigen #2 description (e.g. whether it is a trans-membrane or soluble protein, whether you can provide the recombinant form, etc.)

Do you already have an antibody against target antigen #2?
YesNo
Yesの場合、以下1から8のご質問にお答えください。
1. Specify the method (e.g. hybridoma, phage display, etc.) + exact antigen used for antibody development

2. Specify the species (e.g. human, mouse, etc.) + backbone/format (e.g. IgG1, Fab, scFv, etc.) of the antibody

3. Do you already have its sequence?
YesNo
4-1. Did you already express it as recombinant antibody?
NoYes
4-2. If yes, please specify the format(s) used + backbone species + yield obtained for each format?

5-1. Did you already express it as bispecific antibody?
NoYes
5-2. If yes, please specify the format(s) used + yield obtained for each format?

6. Do you know its epitope?

7. Are you happy with the antibody affinity?

8. Do you have only one antibody against target antigen #2 or several antibodies?

II. Bispecific Antibody Information

What application(s) would you use the bispecific antibody for?

Which disease(s) are you targeting?

What is the expected mechanism of action?

Are effector functions required or not?

Do you have any pharmacokinetics requirement (e.g. regarding half-life, elimination, tissue penetration, etc.)?

Which bispecific format(s) would you be interested in?
Small formats
BiTEDiabodyDARTOther, please specify
IgG-like formats
Knob-into-hole (KiH)CrossMAbDuetMabDuoBodyFab-scFv-FcOther, please specify
Would a patent protection for a specific bispecific format represent a NO-GO for your project? (NB: even if a format is patented, no licensing is needed for preclinical research)
YesNo
Final quantity of purified bispecific antibody required? (NB: exact quantity cannot be guaranteed before small scale pilot production)

What kind of samples will the bispecific antibody be used on? In which animal(s) will you test it?

Endotoxin levels requested

Additional relevant information regarding the final application, target antigen(s), development process, format required…

多くの質問にお答えいただきありがとうございます。

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