弊社が販売する試薬は全て研究用です。ページ内に記載されている料金等の金額は全て参考につき、詳細はお気軽にお問い合わせください。
This service / product is supplied by
& Generay (Shanghai).
目次
qPCR用両末端標識プローブ
蛍光レポーターとクエンチャーの完全アレー
6-FAM, HEX, TETなどの一般的な修飾だけでなく特殊な修飾を含めた合成を提供します。
5’end, 3’endのみならず内部のヌクレオチドへの蛍光色素の直接結合 (オリゴヌクレオチド合成中)
Digoxigenin やいくつかの蛍光色素で直接、内部取り込みができない場合、クロスリンク法で解決します。
二つの分子をクロスリンクする特別な機能グループを使うこと、例えばある種のクロスリンカーとアミン、チオール修飾オリゴヌクレオチドを使用。
オリゴヌクレオチド合成後のクロスリンク法は合成中の修飾に比べ収率が落ちることが知られています。
さらに、すべての合成後の修飾法はHPLC精製を必要とします。PAGEによるそれの精製はより収率を減らします。
ある種の修飾はPAGEにより壊されることもあります。
1. Bio-Synthesisによる合成
- 35ベースまでの価格です。
- 脱保護、脱塩、HPLC精製品
- 幅広い色素とクエンチャーを用意
- QCはMALDI、HPLC、蛍光スキャン
- 納期は約1週間
3′ Black Hole Quencher Dual Labeled Probes | 3′ TAMRA Dual Labeled Probes | 3′ Eclipse Dark Quencher Dual Labeled Probes | 3′ IQ Quencher Dual Labeled Probes | 3′ Blackberry Quencher Dual Labeled Probes and Others
◆3′ Black Hole Quencher Dual Labeled Probes
| 3′ BHQ | Scale | Minimum Guarantee Yield | 参考料金 |
| 5’ TAMRA + 3 ‘BHQ-2™ | 100 nmole scale | 8-10 nmole dual HPLC Purified | 63,000 |
| 250 nmole scale | 20-25 nmole dual HPLC Purified | 76,000 | |
| 1.0 umole scale | 55-60 nmole dual HPLC Purified | 108,000 | |
| 5’ FAM + 3’ BHQ-1™ | 100 nmole scale | 8-10 nmole dual HPLC Purified | 27,000 |
| 250 nmole scale | 20-25 nmole dual HPLC Purified | 45,000 | |
| 1.0 umole scale | 55-60 nmole dual HPLC Purified | 66,000 | |
| 5′ FAM + Internal T-BHQ-1™/ 3′ C3 | 250 nmole | 10-15 nmole HPLC purified | 53,000 |
| 1 umole scale | 30-35 nmole HPLC purified | 93,000 | |
| 5’ HEX + 3’ BHQ-1™ | 100 nmole scale | 8-10 nmole dual HPLC Purified | 42,000 |
| 250 nmole scale | 20-25 nmole dual HPLC Purified | 60,000 | |
| 1.0 umole scale | 55-60 nmole dual HPLC Purified | 86,000 | |
| 5’ TET + 3’ BHQ-1™ | 100 nmole scale | 8-10 nmole dual HPLC Purified | 42,000 |
| 250 nmole scale | 20-25 nmole dual HPLC Purified | 60,000 | |
| 1.0 umole scale | 55-60 nmole dual HPLC Purified | 86,000 | |
| 5’ JOE + 3’ BHQ-1™ | 100 nmole scale | 2-3 nmole dual HPLC purified | 54,000 |
| 250 nmole scale | 8-10 nmole dual HPLC purified | 81,000 | |
| 1.0 umole scale | 35-40 nmole dual HPLC Purified | 108,000 | |
| 5’ ROX + 3’ BHQ-2™ | 100 nmole scale | 2-3 nmole dual HPLC purified | 54,000 |
| 250 nmole scale | 8-10 nmole dual HPLC purified | 76,000 | |
| 1.0 umole scale | 25-30 nmole dual HPLC Purified | 113,000 | |
| 5’ Rhodamine-6G + 3’ BHQ-1™ | 100 nmole scale | 2-3 nmole dual HPLC purified | 54,000 |
| 250 nmole scale | 8-10 nmole dual HPLC purified | 67,000 | |
| 1.0 umole scale | 25-30 nmole dual HPLC Purified | 99,000 | |
| 5’ Texas Red® + 3’ BHQ-2™ | 100 nmole scale | 2-3 nmole dual HPLC purified | 68,000 |
| 250 nmole scale | 8-10 nmole dual HPLC purified | 75,000 | |
| 1.0 umole scale | 35-40 nmole dual HPLC Purified | 108,000 | |
| 5’ Yakima Yellow® + 3’ BHQ-1™ | 100 nmole scale | 5 nmole HPLC Purified | 62,000 |
| 250 nmole scale | 10-15 nmole HPLC Purified | 77,000 | |
| 1.0 umole scale | 45-50 nmole HPLC Purified | 108,000 | |
| 5’ Dragonfly Orang™+ 3’ BHQ-2 ™ | 100 nmole scale | 2-3 nmole dual HPLC purified | 62,000 |
| 250 nmole scale | 8-10 nmole dual HPLC purified | 77,000 | |
| 1.0 umole scale | 25-30 nmole dual HPLC Purified | 108,000 | |
| 5’ Cy® 3 + 3’ BHQ-2™ | 100 nmole scale | 5 nmole HPLC Purified | 53,000 |
| 250 nmole scale | 10-15 nmole HPLC Purified | 67,000 | |
| 1.0 umole scale | 45-50 nmole HPLC Purified | 99,000 | |
| 5’ Cy5® + 3’ BHQ-2™ | 100 nmole scale | 5 nmole HPLC Purified | 53,000 |
| 250 nmole scale | 10-15 nmole HPLC Purified | 67,000 | |
| 1.0 umole scale | 45-50 nmole HPLC Purified | 99,000 | |
| 5’ Cy5® + 3’ BHQ-3™ | 100 nmole scale | 5 nmole HPLC Purified | 117,000 |
| 250 nmole scale | 10-15 nmole HPLC Purified | 153,000 | |
| 1.0 umole scale | 45-50 nmole HPLC Purified | 198,000 | |
| 5’ DYOMICS + 3’ BHQ™ | 100 nmole scale | 2-3 nmole dual HPLC purified | Inquire |
| 250 nmole scale | 8-10 nmole dual HPLC purified | Inquire | |
| 1.0 umole scale | 25-30 nmole dual HPLC Purified | Inquire | |
| 5’ ATTO + 3’ BHQ™ | 100 nmole scale | 2-3 nmole dual HPLC purified | 62,000 |
| 250 nmole scale | 8-10 nmole dual HPLC purified | 76,000 | |
| 1.0 umole scale | 25-30 nmole dual HPLC Purified | 108,000 | |
| 5’ Alexa + 3’ BHQ™ | 100 nmole scale | 2-3 nmole dual HPLC purified | 62,000 |
| 250 nmole scale | 8-10 nmole dual HPLC purified | 76,000 | |
| 1.0 umole scale | 25-30 nmole dual HPLC Purified | 108,000 |
◆3′ TAMRA Dual Labeled Probes
| 3′ TAMRA | Scale | Minimum Guarantee Yield | 参考料金 |
| 5’ FAM + 3′ TAMRA | 100 nmole scale | 8-10 nmole dual HPLC Purified | 40,000 |
| 250 nmole scale | 20-25 nmole dual HPLC Purified | 51,000 | |
| 1.0 umole scale | 55-60 nmole dual HPLC Purified | 66,000 | |
| 5′ TET + 3′ TAMRA | 100 nmole scale | 8-10 nmole dual HPLC Purified | 50,000 |
| 250 nmole scale | 20-25 nmole dual HPLC Purified | 56,000 | |
| 1.0 umole scale | 55-60 nmole dual HPLC Purified | 86,000 | |
| 5′ Yakima Yellow + 3′ TAMRA | 100 nmole scale | 8-10 nmole HPLC Purified | 60,000 |
| 250 nmole scale | 25-30 nmole HPLC Purified | 69,000 | |
| 1.0 umole scale | 55-60 nmole dual HPLC Purified | 103,000 | |
| 5′ HEX + 3′ TAMRA | 100 nmole scale | 8-10 nmole HPLC Purified | 50,000 |
| 250 nmole scale | 20-25 nmole dual HPLC Purified | 68,000 | |
| 1.0 umole scale | 55-60 nmole dual HPLC Purified | 89,000 |
◆3′ Eclipse Dark Quencher Dual Labeled Probes
| 3′ Eclipse Dark Quencher | Scale | 供給量 | 参考料金 |
| 5′ FAM + 3′ Eclipse DQ | 100 nmole | 5 nmole HPLC Purified | 46,000 |
| 250 nmole | 10-15 nmole HPLC Purified | 57,000 | |
| 1.0 umole | 45-50 nmole HPLC Purified | 94,000 | |
| 5′ HEX + 3′ Eclipse DQ | 100 nmole | 5 nmole HPLC Purified | 46,000 |
| 250 nmole | 10-15 nmole HPLC Purified | 57,000 | |
| 1.0 umole | 45-50 nmole HPLC Purified | 94,000 | |
| 5′ TET + 3′ Eclipse DQ | 100 nmole | 5 nmole HPLC Purified | 46,000 |
| 250 nmole | 10-15 nmole HPLC Purified | 57,000 | |
| 1.0 umole | 45-50 nmole HPLC Purified | 94,000 | |
| 5′ Cy5 + 3′ Eclipse DQ | 100 nmole | 5 nmole HPLC Purified | 117,000 |
| 250 nmole | 10-15 nmole HPLC Purified | 153,000 | |
| 1.0 umole | 45-50 nmole HPLC Purified | 198,000 | |
| 5′ Redmond Red | 100 nmole | 5 nmole HPLC Purified | 67,000 |
| 250 nmole | 10-15 nmole HPLC Purified | 78,000 | |
| 1.0 umole | 45-50 nmole HPLC Purified | 108,000 | |
| 5′ JOE + 3′ Eclipse DQ | 100 nmole | 5 nmole HPLC Purified | 58,000 |
| 250 nmole | 10-15 nmole HPLC Purified | 68,000 | |
| 1.0 umole | 45-50 nmole HPLC Purified | 99,000 | |
| 5′ Rhodamine-6G + 3′ Eclipse DQ | 100 nmole | 2-3 nmole dual HPLC purified | 58,000 |
| 250 nmole | 8-10 nmole dual HPLC purified | 68,000 | |
| 1.0 umole | 25-30 nmole dual HPLC Purified | 99,000 | |
| 5′ Yakima Yellow® + 3′ Eclipse DQ | 100 nmole | 5 nmole HPLC Purified | 67,000 |
| 250 nmole | 10-15 nmole HPLC Purified | 78,000 | |
| 1.0 umole | 45-50 nmole HPLC Purified | 108,000 | |
| 5′ DYOMICS + 3′ Eclipse DQ | 100 nmole | 2-3 nmole dual HPLC purified | On Request |
| 250 nmole | 8-10 nmole dual HPLC purified | On Request | |
| 1.0 umole | 25-30 nmole dual HPLC Purified | On Request | |
| 5′ ATTO + 3′ Eclipse DQ | 100 nmole | 2-3 nmole dual HPLC purified | 63,000 |
| 250 nmole | 8-10 nmole dual HPLC purified | 80,000 | |
| 1.0 umole | 25-30 nmole dual HPLC Purified | 106,000 |
◆3′ IQ Quencher Dual Labeled Probes
| 3′ Instant Quencher IQ | Scale | 供給量 | 参考料金 |
| 5′ FAM + IQ2, IQ4 | 100 nmole | 15 nmole HPLC Purified | 35,000 |
| 250 nmole | 25 nmole HPLC Purified | 50,000 | |
| 1.0 umole | 50 nmole HPLC Purified | 75,000 | |
| 5′ HEX + 3′ IQ2 or IQ4 | 100 nmole | 15 nmole HPLC Purified | 44,000 |
| 250 nmole | 25 nmole HPLC Purified | 63,000 | |
| 1.0 umole | 50 nmole HPLC Purified | 94,000 | |
| 5′ TET + 3′ IQ2 or IQ4 | 100 nmole | 15 nmole HPLC Purified | 44,000 |
| 250 nmole | 25 nmole HPLC Purified | 63,000 | |
| 1.0 umole | 50 nmole HPLC Purified | 94,000 | |
| 5′ JOE + 3′ IQ2 or IQ4 | 100 nmole | 15 nmole HPLC Purified | 44,000 |
| 250 nmole | 25 nmole HPLC Purified | 63,000 | |
| 1.0 umole | 50 nmole HPLC Purified | 94,000 | |
| 5′ Cy3 + 3′ IQ4 | 100 nmole | 2 nmole HPLC Purified | 41,000 |
| 250 nmole | 10 nmole HPLC Purified | 47,000 | |
| 1.0 umole | 20 nmole HPLC Purified | 86,000 | |
| 5′ CY5 + 3′ IQ4 | 100 nmole | 2 nmole HPLC Purified | 41,000 |
| 250 nmole | 10 nmole HPLC Purified | 47,000 | |
| 1.0 umole | 20 nmole HPLC Purified | 86,000 | |
| 5′ TAMRA + 3′ IQ4 | 100 nmole | NA | NA |
| 250 nmole | 10 nmole HPLC Purified | 62,000 | |
| 1.0 umole | 20 nmole HPLC Purified | 93,000 | |
| 5′ ROX+ 3′ IQ4 | 100 nmole | NA | NA |
| 250 nmole | 10 nmole HPLC Purified | 76,000 | |
| 1.0 umole | 20 nmole HPLC Purified | 114,000 | |
| 5′ Texas Red + 3′ IQ4 | 100 nmole | NA | NA |
| 250 nmole | 10 nmole HPLC Purified | 76,000 | |
| 1.0 umole | 20 nmole HPLC Purified | 114,000 | |
| 5’ ATTO + 3’ IQ4 | 100 nmole scale | 2-3 nmole dual HPLC purified | 62,000 |
| 250 nmole scale | 8-10 nmole dual HPLC purified | 76,000 | |
| 1.0 umole scale | 25-30 nmole dual HPLC Purified | 108,000 | |
| 5’ Alexa + 3’ IQ4 | 100 nmole scale | 2-3 nmole dual HPLC purified | 62,000 |
| 250 nmole scale | 8-10 nmole dual HPLC purified | 76,000 | |
| 1.0 umole scale | 25-30 nmole dual HPLC Purified | 108,000 |
◆3′ Blackberry Quencher Dual Labeled Probes and Others
| 3′ Blackberry® Quencher (BBQ 650®) | Scale | 供給量 | 参考料金 |
| 5′ JOE + 3′ IQ2 or IQ4 | 100 nmole | 15 nmole HPLC Purified | 44,000 |
| 250 nmole | 25 nmole HPLC Purified | 63,000 | |
| 1.0 umole | 50 nmole HPLC Purified | 94,000 | |
| 5′ Cy3 + 3′ BBQ650 | 100 nmole | 2 nmole HPLC Purified | 41,000 |
| 250 nmole | 10 nmole HPLC Purified | 47,000 | |
| 1.0 umole | 20 nmole HPLC Purified | 86,000 | |
| 5′ CY5 + 3′ BBQ650 | 100 nmole | 2 nmole HPLC Purified | 41,000 |
| 250 nmole | 10 nmole HPLC Purified | 47,000 | |
| 1.0 umole | 20 nmole HPLC Purified | 86,000 | |
| 5′ TAMRA + 3′ BBQ650 | 100 nmole | NA | NA |
| 250 nmole | 10 nmole HPLC Purified | 62,000 | |
| 1.0 umole | 20 nmole HPLC Purified | 93,000 | |
| 5′ ROX+ 3′ BBQ650 | 100 nmole | NA | NA |
| 250 nmole | 10 nmole HPLC Purified | 76,000 | |
| 1.0 umole | 20 nmole HPLC Purified | 114,000 | |
| 5′ Texas Red + 3′ BBQ650 | 100 nmole | NA | NA |
| 250 nmole | 10 nmole HPLC Purified | 76,000 | |
| 1.0 umole | 20 nmole HPLC Purified | 114,000 | |
| 5’ ATTO + 3’ BBQ650 | 100 nmole scale | 2-3 nmole dual HPLC purified | 62,000 |
| 250 nmole scale | 8-10 nmole dual HPLC purified | 76,000 | |
| 1.0 umole scale | 25-30 nmole dual HPLC Purified | 108,000 | |
| 5’ Alexa + 3’ BBQ650 | 100 nmole scale | 2-3 nmole dual HPLC purified | 62,000 |
| 250 nmole scale | 8-10 nmole dual HPLC purified | 76,000 | |
| 1.0 umole scale | 25-30 nmole dual HPLC Purified | 108,000 |
2. Generayによる合成
ご注意: 以下は修飾料金です。長さに応じ、基本合成料金がかかります。
| Dual-Labeled DNA Probes | ||
| 5′ 6-FAM – 3’TAMRA | Yield(10-60base) | 参考料金 |
| G010-FT | 2 ODs | 16,000 |
| G020-FT | 5 ODs | 21,400 |
| G040-FT | 10 ODs | 32,000 |
| G100-FT | 20 ODs | 48,000 |
| 5′ TET – 3’TAMRA | Yield(10-60base) | 参考料金 |
| G010-TT | 2 ODs | 21,400 |
| G020-TT | 5 ODs | 26,500 |
| 5′ HEX – 3’TAMRA | Yield(10-60base) | 参考料金 |
| G010-HT | 2 ODs | 21,400 |
| G020-HT | 5 ODs | 26,500 |
| 5′ JOE – 3’TAMRA | Yield(10-60base) | 参考料金 |
| G010-JT | 2 ODs | 21,400 |
| G020-JT | 5 ODs | 26,500 |
| 5′ 6-FAM – 3’BHQ1 | Yield(10-60base) | 参考料金 |
| G010-FB | 2 ODs | 16,000 |
| G020-FB | 5 ODs | 21,400 |
| G040-FB | 10 ODs | 32,000 |
| G100-FB | 20 ODs | 44,700 |
| 5′ TET – 3’BHQ1 | Yield(10-60base) | 参考料金 |
| G010-TB | 2 ODs | 16,000 |
| G020-TB | 5 ODs | 21,400 |
| 5′ HEX – 3’BHQ1 | Yield(10-60base) | 参考料金 |
| G010-HB | 2 ODs | 16,000 |
| G020-HB | 5 ODs | 21,400 |
| 5′ JOE – 3’BHQ1 | Yield(10-60base) | 参考料金 |
| G010-JB | 2 ODs | 16,000 |
| G020-JB | 5 ODs | 21,400 |
| 5’ROX – 3’BHQ-2 | Yield(10-60base) | 参考料金 |
| G010-RB | 2 ODs | 21,400 |
| G020-RB | 5 ODs | 28,500 |
| 5’CY3 – 3’BHQ-2 | Yield(10-60base) | 参考料金 |
| G010-CY3B | 2 ODs | 26,700 |
| G020-CY3B | 5 ODs | 40,000 |
| 5’CY5 – 3’BHQ-2 | Yield(10-60base) | 参考料金 |
| G010-CY5B | 2 ODs | 26,700 |
| G020-CY5B | 5 ODs | 40,000 |