qPCR(リアルタイムPCR)プローブ・プライマー合成

オリゴヌクレオチド合成トップページ 関連製品・サービス お問い合わせ・ご注文

目次

概 要 Bio-Synthesis社 Generay社

qPCR Probes by Bio-Synthesis Inc (Texas) & Generay (Shanghai)

qPCR用両末端標識プローブ

蛍光レポーターとクエンチャーの完全アレー

6-FAM, HEX, TETなどの一般的な修飾だけでなく特殊な修飾を含めた合成を提供します。
5’end, 3’endのみならず内部のヌクレオチドへの蛍光色素の直接結合 (オリゴヌクレオチド合成中)
Digoxigenin やいくつかの蛍光色素で直接、内部取り込みができない場合、クロスリンク法で解決します。
二つの分子をクロスリンクする特別な機能グループを使うこと、例えばある種のクロスリンカーとアミン、チオール修飾オリゴヌクレオチドを使用。
オリゴヌクレオチド合成後のクロスリンク法は合成中の修飾に比べ収率が落ちることが知られています。
さらに、すべての合成後の修飾法はHPLC精製を必要とします。PAGEによるそれの精製はより収率を減らします。
ある種の修飾はPAGEにより壊されることもあります。

以下に表記の料金は参考です。お気軽にお問い合わせください。

  • 35ベースまでの価格です。
  • 脱保護、脱塩、HPLC精製品
  • 幅広い色素とクエンチャーを用意
  • QCはMALDI、HPLC、蛍光スキャン
  • 納期は約1週間

3′ Black Hole Quencher Dual Labeled Probes | 3′ TAMRA Dual Labeled Probes | 3′ Eclipse Dark Quencher Dual Labeled Probes | 3′ IQ Quencher Dual Labeled Probes | 3′ Blackberry Quencher Dual Labeled Probes and Others

◆3′ Black Hole Quencher Dual Labeled Probes

3′ BHQ Scale Minimum Guarantee Yield 参考料金
5’ TAMRA + 3 ‘BHQ-2™ 100 nmole scale 8-10 nmole dual HPLC Purified 63,000
250 nmole scale 20-25 nmole dual HPLC Purified 76,000
1.0 umole scale 55-60 nmole dual HPLC Purified 108,000
5’ FAM + 3’ BHQ-1™ 100 nmole scale 8-10 nmole dual HPLC Purified 27,000
250 nmole scale 20-25 nmole dual HPLC Purified 45,000
1.0 umole scale 55-60 nmole dual HPLC Purified 66,000
5′ FAM + Internal T-BHQ-1™/ 3′ C3 250 nmole 10-15 nmole HPLC purified 53,000
1 umole scale 30-35 nmole HPLC purified 93,000
5’ HEX + 3’ BHQ-1™ 100 nmole scale 8-10 nmole dual HPLC Purified 42,000
250 nmole scale 20-25 nmole dual HPLC Purified 60,000
1.0 umole scale 55-60 nmole dual HPLC Purified 86,000
5’ TET + 3’ BHQ-1™ 100 nmole scale 8-10 nmole dual HPLC Purified 42,000
250 nmole scale 20-25 nmole dual HPLC Purified 60,000
1.0 umole scale 55-60 nmole dual HPLC Purified 86,000
5’ JOE + 3’ BHQ-1™ 100 nmole scale 2-3 nmole dual HPLC purified 54,000
250 nmole scale 8-10 nmole dual HPLC purified 81,000
1.0 umole scale 35-40 nmole dual HPLC Purified 108,000
5’ ROX + 3’ BHQ-2™ 100 nmole scale 2-3 nmole dual HPLC purified 54,000
250 nmole scale 8-10 nmole dual HPLC purified 76,000
1.0 umole scale 25-30 nmole dual HPLC Purified 113,000
5’ Rhodamine-6G + 3’ BHQ-1™ 100 nmole scale 2-3 nmole dual HPLC purified 54,000
250 nmole scale 8-10 nmole dual HPLC purified 67,000
1.0 umole scale 25-30 nmole dual HPLC Purified 99,000
5’ Texas Red® + 3’ BHQ-2™ 100 nmole scale 2-3 nmole dual HPLC purified 68,000
250 nmole scale 8-10 nmole dual HPLC purified 75,000
1.0 umole scale 35-40 nmole dual HPLC Purified 108,000
5’ Yakima Yellow® + 3’ BHQ-1™ 100 nmole scale 5 nmole HPLC Purified 62,000
250 nmole scale 10-15 nmole HPLC Purified 77,000
1.0 umole scale 45-50 nmole HPLC Purified 108,000
5’ Dragonfly Orang™+ 3’ BHQ-2 ™ 100 nmole scale 2-3 nmole dual HPLC purified 62,000
250 nmole scale 8-10 nmole dual HPLC purified 77,000
1.0 umole scale 25-30 nmole dual HPLC Purified 108,000
5’ Cy® 3 + 3’ BHQ-2™ 100 nmole scale 5 nmole HPLC Purified 53,000
250 nmole scale 10-15 nmole HPLC Purified 67,000
1.0 umole scale 45-50 nmole HPLC Purified 99,000
5’ Cy5® + 3’ BHQ-2™ 100 nmole scale 5 nmole HPLC Purified 53,000
250 nmole scale 10-15 nmole HPLC Purified 67,000
1.0 umole scale 45-50 nmole HPLC Purified 99,000
5’ Cy5® + 3’ BHQ-3™ 100 nmole scale 5 nmole HPLC Purified 117,000
250 nmole scale 10-15 nmole HPLC Purified 153,000
1.0 umole scale 45-50 nmole HPLC Purified 198,000
5’ DYOMICS + 3’ BHQ™ 100 nmole scale 2-3 nmole dual HPLC purified Inquire
250 nmole scale 8-10 nmole dual HPLC purified Inquire
1.0 umole scale 25-30 nmole dual HPLC Purified Inquire
5’ ATTO + 3’ BHQ™ 100 nmole scale 2-3 nmole dual HPLC purified 62,000
250 nmole scale 8-10 nmole dual HPLC purified 76,000
1.0 umole scale 25-30 nmole dual HPLC Purified 108,000
5’ Alexa + 3’ BHQ™ 100 nmole scale 2-3 nmole dual HPLC purified 62,000
250 nmole scale 8-10 nmole dual HPLC purified 76,000
1.0 umole scale 25-30 nmole dual HPLC Purified 108,000

◆3′ TAMRA Dual Labeled Probes

3′ TAMRA Scale Minimum Guarantee Yield 参考料金
5’ FAM + 3′ TAMRA 100 nmole scale 8-10 nmole dual HPLC Purified 40,000
250 nmole scale 20-25 nmole dual HPLC Purified 51,000
1.0 umole scale 55-60 nmole dual HPLC Purified 66,000
5′ TET + 3′ TAMRA 100 nmole scale 8-10 nmole dual HPLC Purified 50,000
250 nmole scale 20-25 nmole dual HPLC Purified 56,000
1.0 umole scale 55-60 nmole dual HPLC Purified 86,000
5′ Yakima Yellow + 3′ TAMRA 100 nmole scale 8-10 nmole HPLC Purified 60,000
250 nmole scale 25-30 nmole HPLC Purified 69,000
1.0 umole scale 55-60 nmole dual HPLC Purified 103,000
5′ HEX + 3′ TAMRA 100 nmole scale 8-10 nmole HPLC Purified 50,000
250 nmole scale 20-25 nmole dual HPLC Purified 68,000
1.0 umole scale 55-60 nmole dual HPLC Purified 89,000

◆3′ Eclipse Dark Quencher Dual Labeled Probes

3′ Eclipse Dark Quencher Scale 供給量 参考料金
5′ FAM + 3′ Eclipse DQ 100 nmole 5 nmole HPLC Purified 46,000
250 nmole 10-15 nmole HPLC Purified 57,000
1.0 umole 45-50 nmole HPLC Purified 94,000
5′ HEX + 3′ Eclipse DQ 100 nmole 5 nmole HPLC Purified 46,000
250 nmole 10-15 nmole HPLC Purified 57,000
1.0 umole 45-50 nmole HPLC Purified 94,000
5′ TET + 3′ Eclipse DQ 100 nmole 5 nmole HPLC Purified 46,000
250 nmole 10-15 nmole HPLC Purified 57,000
1.0 umole 45-50 nmole HPLC Purified 94,000
5′ Cy5 + 3′ Eclipse DQ 100 nmole 5 nmole HPLC Purified 117,000
250 nmole 10-15 nmole HPLC Purified 153,000
1.0 umole 45-50 nmole HPLC Purified 198,000
5′ Redmond Red 100 nmole 5 nmole HPLC Purified 67,000
250 nmole 10-15 nmole HPLC Purified 78,000
1.0 umole 45-50 nmole HPLC Purified 108,000
5′ JOE + 3′ Eclipse DQ 100 nmole 5 nmole HPLC Purified 58,000
250 nmole 10-15 nmole HPLC Purified 68,000
1.0 umole 45-50 nmole HPLC Purified 99,000
5′ Rhodamine-6G + 3′ Eclipse DQ 100 nmole 2-3 nmole dual HPLC purified 58,000
250 nmole 8-10 nmole dual HPLC purified 68,000
1.0 umole 25-30 nmole dual HPLC Purified 99,000
5′ Yakima Yellow® + 3′ Eclipse DQ 100 nmole 5 nmole HPLC Purified 67,000
250 nmole 10-15 nmole HPLC Purified 78,000
1.0 umole 45-50 nmole HPLC Purified 108,000
5′ DYOMICS + 3′ Eclipse DQ 100 nmole 2-3 nmole dual HPLC purified On Request
250 nmole 8-10 nmole dual HPLC purified On Request
1.0 umole 25-30 nmole dual HPLC Purified On Request
5′ ATTO + 3′ Eclipse DQ 100 nmole 2-3 nmole dual HPLC purified 63,000
250 nmole 8-10 nmole dual HPLC purified 80,000
1.0 umole 25-30 nmole dual HPLC Purified 106,000

◆3′ IQ Quencher Dual Labeled Probes

3′ Instant Quencher IQ Scale 供給量 参考料金
5′ FAM + IQ2, IQ4 100 nmole 15 nmole HPLC Purified 35,000
250 nmole 25 nmole HPLC Purified 50,000
1.0 umole 50 nmole HPLC Purified 75,000
5′ HEX + 3′ IQ2 or IQ4 100 nmole 15 nmole HPLC Purified 44,000
250 nmole 25 nmole HPLC Purified 63,000
1.0 umole 50 nmole HPLC Purified 94,000
5′ TET + 3′ IQ2 or IQ4 100 nmole 15 nmole HPLC Purified 44,000
250 nmole 25 nmole HPLC Purified 63,000
1.0 umole 50 nmole HPLC Purified 94,000
5′ JOE + 3′ IQ2 or IQ4 100 nmole 15 nmole HPLC Purified 44,000
250 nmole 25 nmole HPLC Purified 63,000
1.0 umole 50 nmole HPLC Purified 94,000
5′ Cy3 + 3′ IQ4 100 nmole 2 nmole HPLC Purified 41,000
250 nmole 10 nmole HPLC Purified 47,000
1.0 umole 20 nmole HPLC Purified 86,000
5′ CY5 + 3′ IQ4 100 nmole 2 nmole HPLC Purified 41,000
250 nmole 10 nmole HPLC Purified 47,000
1.0 umole 20 nmole HPLC Purified 86,000
5′ TAMRA + 3′ IQ4 100 nmole NA NA
250 nmole 10 nmole HPLC Purified 62,000
1.0 umole 20 nmole HPLC Purified 93,000
5′ ROX+ 3′ IQ4 100 nmole NA NA
250 nmole 10 nmole HPLC Purified 76,000
1.0 umole 20 nmole HPLC Purified 114,000
5′ Texas Red + 3′ IQ4 100 nmole NA NA
250 nmole 10 nmole HPLC Purified 76,000
1.0 umole 20 nmole HPLC Purified 114,000
5’ ATTO + 3’ IQ4 100 nmole scale 2-3 nmole dual HPLC purified 62,000
250 nmole scale 8-10 nmole dual HPLC purified 76,000
1.0 umole scale 25-30 nmole dual HPLC Purified 108,000
5’ Alexa + 3’ IQ4 100 nmole scale 2-3 nmole dual HPLC purified 62,000
250 nmole scale 8-10 nmole dual HPLC purified 76,000
1.0 umole scale 25-30 nmole dual HPLC Purified 108,000

◆3′ Blackberry Quencher Dual Labeled Probes and Others

3′ Blackberry® Quencher (BBQ 650®) Scale 供給量 参考料金
5′ JOE + 3′ IQ2 or IQ4 100 nmole 15 nmole HPLC Purified 44,000
250 nmole 25 nmole HPLC Purified 63,000
1.0 umole 50 nmole HPLC Purified 94,000
5′ Cy3 + 3′ BBQ650 100 nmole 2 nmole HPLC Purified 41,000
250 nmole 10 nmole HPLC Purified 47,000
1.0 umole 20 nmole HPLC Purified 86,000
5′ CY5 + 3′ BBQ650 100 nmole 2 nmole HPLC Purified 41,000
250 nmole 10 nmole HPLC Purified 47,000
1.0 umole 20 nmole HPLC Purified 86,000
5′ TAMRA + 3′ BBQ650 100 nmole NA NA
250 nmole 10 nmole HPLC Purified 62,000
1.0 umole 20 nmole HPLC Purified 93,000
5′ ROX+ 3′ BBQ650 100 nmole NA NA
250 nmole 10 nmole HPLC Purified 76,000
1.0 umole 20 nmole HPLC Purified 114,000
5′ Texas Red + 3′ BBQ650 100 nmole NA NA
250 nmole 10 nmole HPLC Purified 76,000
1.0 umole 20 nmole HPLC Purified 114,000
5’ ATTO + 3’ BBQ650 100 nmole scale 2-3 nmole dual HPLC purified 62,000
250 nmole scale 8-10 nmole dual HPLC purified 76,000
1.0 umole scale 25-30 nmole dual HPLC Purified 108,000
5’ Alexa + 3’ BBQ650 100 nmole scale 2-3 nmole dual HPLC purified 62,000
250 nmole scale 8-10 nmole dual HPLC purified 76,000
1.0 umole scale 25-30 nmole dual HPLC Purified 108,000

2. Generay

ご注意: 修飾料金です。長さに応じ、基本合成料金がかかります。

Dual-Labeled DNA Probes
5′ 6-FAM – 3’TAMRA Yield(10-60base) 参考料金
G010-FT 2 ODs 16,000
G020-FT 5 ODs 21,400
G040-FT 10 ODs 32,000
G100-FT 20 ODs 48,000
5′ TET – 3’TAMRA Yield(10-60base) 参考料金
G010-TT 2 ODs 21,400
G020-TT 5 ODs 26,500
5′ HEX – 3’TAMRA Yield(10-60base) 参考料金
G010-HT 2 ODs 21,400
G020-HT 5 ODs 26,500
5′ JOE – 3’TAMRA Yield(10-60base) 参考料金
G010-JT 2 ODs 21,400
G020-JT 5 ODs 26,500
5′ 6-FAM – 3’BHQ1 Yield(10-60base) 参考料金
G010-FB 2 ODs 16,000
G020-FB 5 ODs 21,400
G040-FB 10 ODs 32,000
G100-FB 20 ODs 44,700
5′ TET – 3’BHQ1 Yield(10-60base) 参考料金
G010-TB 2 ODs 16,000
G020-TB 5 ODs 21,400
5′ HEX – 3’BHQ1 Yield(10-60base) 参考料金
G010-HB 2 ODs 16,000
G020-HB 5 ODs 21,400
5′ JOE – 3’BHQ1 Yield(10-60base) 参考料金
G010-JB 2 ODs 16,000
G020-JB 5 ODs 21,400
5’ROX – 3’BHQ-2 Yield(10-60base) 参考料金
G010-RB 2 ODs 21,400
G020-RB 5 ODs 28,500
5’CY3 – 3’BHQ-2 Yield(10-60base) 参考料金
G010-CY3B 2 ODs 26,700
G020-CY3B 5 ODs 40,000
5’CY5 – 3’BHQ-2 Yield(10-60base) 参考料金
G010-CY5B 2 ODs 26,700
G020-CY5B 5 ODs 40,000

qPCRプローブのお問い合わせ・ご注文

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下記フォームからご送信されるか、あるいは biologic [at] rr.iij4u.or.jp へメールでお送り頂いても結構です。

  • 塩基の表記例
    ・DNA bases: A, G, C, T
    ・RNA bases: rA, rG, rC, rU
    ・2'-O-methyl RNA bases: mA, mG, mC, mU
    ・2' Fluoro RNA bases: fA, fG, fC, fU
    ・Phosphorothioate bond: *
  • 配列の表記例
    ・DNA, PS = 5′ T*C*A*T*G*C*G*A*T*A*T*G*T*C*C*A*T*C*G*A 3′
    ・DNA/RNA mix, PS/PO chimera = 5′ T*G*G*TGCrGArArATGTCCAT*C*G*T 3′
    ・2′OMe/DNA chimera, PS = 5′ mU*mC*mC*mU*mG*C*G*A*A*A*T*G*T*C*C*mA*mU*mC*mG*mU 3′


オリゴ合成
見積もりご注文



電話番号 Phone number

本数 Number

種類 Type
DNARNAその他

製品名 Code name
P.S.Ultra SUltra 1Ultra 2G.R.その他

精製度 Purification
ゲルろ過(脱塩)カートリッジ (OPC)HPLCPAGEその他


*お願い：1営業日以内に返信メールが届かない場合は、大変お手数ですがbiologic [at] rr.iij4u.or.jpへ直接メールをお送りください。