受託分析 次世代シーケンシング (NGS) [ChunLab]


ChunLab NGS Service Price List

Microbiome (16S /ITS gene) Full Service

Service includes:

  • DNA sample QC
  • Library construction
  • Roche 454 or MiSeq 16S amplicon sequencing
  • Comprehensive data analysis
  • Creation of CLC format files to be browsed and further analyzed in ChunLab’s CLcommunity software
  • CLcommunity software included free
種類 10 or less samples 11-20 21-50
454 Junior, 5K reads 26,000円/sample 22,800円 21,500円
MiSeq Nano Flow Cell
(250bp PE), 20K reads
21,000/sample 19,500 17,600

RNA Sequencing Full Service

Service includes:

  • Sample QC
  • Strand specific library construction (rRNA depletion for Bacterial samples only)
  • Illumina HiSeq 2500 sequencing
  • Comprehensive data analysis
  • Creation of CLT and CLG format files to be browsed and further analyzed in ChunLab’s CLRNASeq software
  • CLRNASeq software included free
種類 4 or less samples 5-12 13+
Bacteria, 50bp SE 156,000円/sample 148,000円 130,000円
Eukaryote, 100bp PE 169,000/sample 163,000 149,000

Bacterial Genome De novo Sequencing and Data Analysis

Service includes:

  • Sample QC
  • MiSeq 300bp PE sequencing; >100X coverage
  • PacBio C4-P6, >70X coverage
  • Comprehensive data analysis
  • Creation of CLG and CG format files to be browsed and further analyzed in ChunLab’s CLgenomics software
  • CLgenomics software included free
MiSeq 300bp PE sequencing; >100X coverage 156,000円
PacBio C4-P6, >70X coverage 364,000円
Resequencing MiSeq 117,000円

Bioinformatics Only Services

Microbiome (16S /ITS gene) Bioinformatics Analysis with Software
Analysis includes:

  • Superior phylogenetic identification and taxonomic assignment of each read at the species level based on the EzTaxon and EzFungi databases
  • Alpha and beta diversity indices, rarefaction curves, rank abundance, environmental correlations, source tracking, clustering, UniFrac multivariate analysis, canonical correspondence analysis
  • Creation of CLC format files to be browsed and further analyzed in ChunLab’s CLcommunity software
  • CLcommunity software included free

Analysis for any platform Analysis not possible if LT 16S metagenomic kit is used

長さ <10 samples 11-20 21-50 51-100 101-250 >250
<50K 13,000円/sample 11,000円 7,800円 6,500円 5,200円 3,900円
51-100K 15,000/sample 13,000 9,800 7,800 6,500 4,600
101-200K 17,600/sample 15,000 11,000 9,800 7,800 5,900
201K-300K 20,200/sample 17,600 13,000 11,700 9,100 7,200

RNA Sequencing Bioinformatics Analysis with Software

Analysis includes:

  • Mapping of transcript expressions (of multiple samples) to reference genome of client’s choice
  • Differential gene expression analysis between any two selected samples (DEG Browser)
  • KEGG, eggnog and gene ontology metabolic and pathway enrichment analyses
  • Clustering of gene groups with similar expression patterns
  • SNP / InDel detection
  • Creation of CLT format files to be browsed and further analyzed in ChunLab’s CLRNASeq software
  • CLRNASeq software included free
サンプル数 1~3 4~9 10~29 30~49 50~100
料金 31,200円/sample 29,300円 26,000円 22,800円 19,500円

Microbial Genome Analysis Bioinformatics Only

Analysis Includes:

  • Assembly
  • Annotation (SEED, RefSeq, Subsystems, COG and KEGG)
  • Plus 3 comparative genome analysis (gene by gene level comparison)
  • Creation of CLG and CG format files to be browsed and further analyzed in ChunLab’s CLgenomics software
  • CLgenomics software included free

De novo (genome size up to 5MB)

  • 39,000円 for assembly*
  • 52,000円 for annotation
  • 39,000円 SNV/SNP Analysis (analysis provided in Excel) *assembly price increase by 13,000円 per 1MB

Comparative Genomics

  • 26,000円 for 3 genomes
  • 39,000円 for 5 genomes
  • 65,000円 for 10 genomes
  • 130,000円 for 20 genomes

Non NGS Services Description Pricing

Bionano Irys Map Service

Service Includes:

  • DNA Plug QC
  • Library Creation
  • Mapping using Irys Chip
  • Irys View Software for viewing map, uploading NGS data for alignment and scaffolding, and comparison on different Irys maps

One Irys chip contains 2 lanes One lane produces 10-50GB of data

Component pricing

  • 11,700円 – Plug QC
  • 70,000円 – library
  • 320,000円 per chip *As plug preparation is tedious, recommend clients to submit 5 plugs at one time **Coverage of 50X is minimum but recommend 100X for best results

nCounter Digital Count Profiling

Service Includes:

  • Sample QC
  • nCounter Analysis
  • Data analysis – results delivered in Excel / Report format