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EasyPure® Genomic DNA Kit provides a simple and convenient way to isolate high quality genomic DNA from a variety of mammalian cells, tissues, E.coli and yeast. Cells and tissues are enzymatically lysed. DNA is bound to silica-based column.
|50 rxns (with RNase A)||200 rxns (with RNase A)|
|50 rxns||200 rxns|
- DNA yield up to 15 μg.
- Complete removal of contaminants and inhibitors.
- Column based purification, no organic extraction or ethanol precipitation.
- Restriction enzyme digestion
- Southern blot
RNase A and Proteinase K solutions at -20°C for one year; others at room temperature for one year
|Component||EE101-01 (50 rxns)||EE101-02 (200 rxns)|
|EE101-11 (50 rxns)||EE101-12 (200 rxns)|
|Lysis Buffer 2 (LB2)||6 ml||24 ml|
|Wash Buffer 2 (WB2)||12 ml||2×22 ml|
|Binding Buffer 2 (BB2)||28 ml||110 ml|
|Clean Buffer 2 (CB2)||55 ml||2×110 ml|
|Elution Buffer (EB)||25 ml||80 ml|
|RNase A (20 mg/ml)||1 ml (EE101-01)||4×1 ml (EE101-02)|
|0 (EE101-11)||0 (EE101-12)|
|Proteinase K (20 mg/ml)||1 ml||4×1 ml|
|Genomic Spin Column with Collection Tubes||50 each||200 each|