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|詳細 ･ 特長
|I-O™ Mesenchymal Stem Cell Media, Xeno-Free (XF) (CAT#: ITS-0622-YT18)
|This medium is optimized for the expansion of human mesenchymal stem cells. There is purified Human Serum Albumin (HSA) in the media. It is is ready to use or it may be used with additional cytokine/growth factor supplements for the desired cell culture application. MSCs grown under Xeno-Free (XF) conditions should be grown on extracellular matrix (ECM) protein-coated plates.
|I-O™ Essential 8 Medium Kit (CAT#: ITS-0622-YT356)
|I-O™ Essential 8 Medium is a Xeno-Free (XF) and feeder-free medium specially formulated for the growth and expansion of human pluripotent stem cells (PSCs). I-O™ Essential 8 Medium Kit contains E8 Basal medium and 25X E8 Supplement, providing a simpler medium with low-protein formulation for the culture of pluripotent stem cells. This kit has been tested on human embryonic stem (ES) cells and human induced pluripotent stem (iPS) cells. iPSCs maintain growth without any signs of karyotypic abnormalities and maintain differentiation of all three germ line lineages.
|詳細 ･ 特長
|I-O™ Mesenchymal Stem Cell Chondrogenic Differentiation Media (CAT#: ITS-0622-YT21)
|This medium is a base medium for the differentiation of MSCs into chondrocytes. All the components have been selected and optimized for human, mouse, and rat MSC chondrogenesis. This medium requires supplements (not included), such as human/mouse/rat chondrogenic supplement or user-defined cytokines and growth factors to induce chondrogenesis. Differentiation to mature chondrocytes is seen between 14 and 28 days.
|I-O™ hMSCs Osteogenic Differentiation Medium (CAT#: ITS-0622-YT42)
|This medium is a Serum-Free (SF) complete medium optimized for the osteogenic differentiation of human adipose-derived mesenchymal stem/stromal cells (MSCs) and human amniotic fluid stem cells (AFSCs). It is ready to use and can be supplemented with additional cytokines/ growth factors for desired applications. Differentiation to mature chondrocytes is seen between 14 and 21 days.
|I-O™ Mesenchymal Stem Cell Osteogenic/Adipogenic Differentiation Media (CAT#: ITS-0622-YT20)
|Mesenchymal stem cells (MSCs) can differentiate into adipocytes, osteoblasts, myocytes, and chondrocytes in vivo and in vitro. This medium is a base medium for the differentiation of MSCs into osteocytes or adipocytes. Creative Biolabs has developed a series of mediums and supplements for cell culture, expansion and differentiation. This medium requires supplements (not included), such as osteogenic supplement, adipogenic supplement, or user-selected cytokines and growth factors. Differentiation to mature osteocytes/adipocytes is seen between 2-3 weeks.
|詳細 ･ 特長
|Y 27632 dihydrochloride
Specific inhibitor of Rho-associated ROCK kinases which supresses tumour cell invasion and affects cell proliferation, differentiation and motility. The compound competes with ATP for binding to the kinase catalytic site and inhibits phosphorylation of ROCK substrates. This inhibitor was also shown to attenuate neuronal death after transient retinal ischemia and to possess protective effects on human embryonic stem cells.
|詳細 ･ 特長
Inhibitor of activin receptor-like kinases (ALK4, ALK5 and ALK7), members of TGFβ type I receptor family. Blocks activin- and TGFβ-mediated signaling and phosphorylation of Smad2 downstream. Suppresses tumor-promoting and tumor-suppressing effects of TGFβ. Promotes differentiation of human embryonic stem cells (hESCs), whilst sustaining mouse ESCs in the undifferentiated state.
|詳細 ･ 特長
Potent and selective inhibitor of the glycogen synthase kinase GSK-3. Promotes cell renewal in embryonic stem cells by modulating Notch, MAPK and TGF-β signalling pathways, which are involved in pluripotency. It also affects the Wnt/β-catenin pathway and alters expression of genes coding for proteins, epigenetic regulatory elements and non-coding RNAs. The combination of CHIR 99021 and basic fibroblast growth factor (bFGF) allows long-term self-renewal in adult mouse retinal progenitor cells.