pEASY®-Blunt E1 Expression Kit : CE111


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pEASY®-Blunt E1 Expression Vector is constructed from pET vector, it utilizes a highly efficient, five-minute blunt cloning strategy to clone PCR product into high-efficient expression vector. The size of control insert is 750 bp, and expressed target protein is about 27 kDa.




TransGen Biotech社製品の参考価格


10 rxns


  • 5 minutes fast ligation of Pfu-amplified PCR products.
  • Ampicillin resistance.
  • T7 promoter primer and T7 terminator primer for sequencing.
  • Bacteriophage T7lac promoter for high level expression.
  • N-terminal 6×His tag for easy purification.
  • Trans1-T1 phage resistant chemically competent cell, high transformation efficiency (>109 cfu/μg pUC19 DNA) and fast growing.
  • E1 Expression Plasmid included as negative control.


Competent Cell at -70°C for six months; others at -20°C for nine months


pEASY®-Blunt E1 Expression Vector (15 ng/μl) 10 µl
E1 Expression Plasmid (Negative Control) (15 ng/μl) 10 µl
EControl Template (5 ng/μl) 10 µl
EControl Forward Primer (10 μM) 10 µl
EControl Reverse Primer (10 μM) 10 µl
T7 Promoter Primer (10 μM) 50 µl
T7 Terminator Primer (10 μM) 50 µl
Trans1-T1 Phage Resistant Chemically Competent Cell 5×100 μl


Journal IF Author Date Link
A Viral Satellite DNA Vector (TYLCCNV) for Functional Analysis of miRNAs and siRNAs in Plants
Plant Physiology 7.367 Dongyan Cao, et al. College of Food Science and Nu 2017 Apr The original link
Identification of a novel class B scavenger receptor homologue in Portunus trituberculatus: Molecular cloning and microbial ligand binding
fish & shellfish immunology 3.025 Yang N, et al. Key Laboratory of the Ministry of E 2016 Nov The original link
Genome-wide identification and functional analysis of S-RNase involved in the self-incompatibility of citrus
molecular genetics and genomics 2.622 Liang M, et al. Key Laboratory of Horticultural Pl 2016 Dec The original link
Identification and functional characterization of an odorant receptor in pea aphid, Acyrthosiphon pisum
insect science 2.026 Rui-Bin Zhang,et al. 2017Jul The original link
Characterization of a neutral recombinant xylanase from Thermoactinospora rubra YIM 77501T
antonie van leeuwenhoek international journal of g 1.944 Yin YR, et al. Yunnan Institute of Microbiology, Y 2017 Mar The original link

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